Detection of circulating Yersinia-immunoglobulin complexes by enzyme immunoassay (EIA)

J Immunol Methods. 1986 May 22;89(2):191-9. doi: 10.1016/0022-1759(86)90357-1.


An enzyme immunoassay (EIA) was developed for the detection of Yersinia-immunoglobulin complexes of known Ig class. Immune complexes (ICs) were attached to polystyrene microtiter plates by rabbit anti-human immunoglobulins, and the existence of Yersinia enterocolitica O:3 antigens was demonstrated using Fab fragments of alkaline phosphatase (AP)-conjugated antibody against the same serotype. Simultaneous binding of Yersinia antigens and immunoglobulins was a prerequisite for the detection of ICs. The method will be valuable for research into the immunopathogenetic mechanisms leading to reactive arthritis after Yersinia infection.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Bacterial / analysis*
  • Antibody Specificity
  • Antigen-Antibody Complex / analysis*
  • Follow-Up Studies
  • Humans
  • Immunoenzyme Techniques
  • Immunoglobulins / analysis*
  • Rabbits
  • Rheumatoid Factor / analysis
  • Salmonella / immunology
  • Yersinia Infections / immunology
  • Yersinia enterocolitica / immunology*


  • Antibodies, Bacterial
  • Antigen-Antibody Complex
  • Immunoglobulins
  • Rheumatoid Factor