Arrested crossover precursor structures form stable homologous bonds in a Tetrahymena meiotic mutant

Miao Tian; Kazufumi Mochizuki; Josef Loidl

doi:10.1371/journal.pone.0263691

Arrested crossover precursor structures form stable homologous bonds in a Tetrahymena meiotic mutant

PLoS One. 2022 Feb 16;17(2):e0263691. doi: 10.1371/journal.pone.0263691. eCollection 2022.

Authors

Miao Tian¹, Kazufumi Mochizuki², Josef Loidl¹

Affiliations

¹ Department of Chromosome Biology, Max Perutz Labs, University of Vienna, Vienna, Austria.
² IGH (Institute of Human Genetics), Montpellier, France.

Abstract

Meiotic DNA double-strand breaks produce reciprocally exchanged DNA strands, which mature into chiasmata that hold homologous chromosomes together as bivalents. These bivalents are subsequently separated in the first meiotic division. In a mutant lacking the newly identified Tetrahymena gene APRO1 (Anaphase promoting 1), meiosis is arrested by the end of prophase. Mature chiasmata are not formed but bivalents are connected via a molecular precursor structure. In-depth analysis of this arrested intermediate structure may help to elucidate the noncanonical molecular recombination pathway in Tetrahymena.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosomes / genetics*
DNA Breaks, Double-Stranded*
DNA Repair*
Homologous Recombination*
Meiosis*
Tetrahymena / genetics*

Grants and funding

This work was supported by grant P27313-B20 from the Austrian Science Fund (www.fwf.ac.at/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.