Modeling Breast Cancer in Organoid and Intraductal Models

Richard Iggo

doi:10.1007/978-1-0716-2193-6_13

Modeling Breast Cancer in Organoid and Intraductal Models

Methods Mol Biol. 2022:2471:235-257. doi: 10.1007/978-1-0716-2193-6_13.

Author

Richard Iggo^{1

2}

Affiliations

¹ INSERM U1218, Institut Bergonié, University of Bordeaux, Bordeaux, France. richard.iggo@u-bordeaux.fr.
² DRMCRL Lab, University of Adelaide Medical School, Adelaide, SA, Australia. richard.iggo@u-bordeaux.fr.

PMID: 35175601
DOI: 10.1007/978-1-0716-2193-6_13

Abstract

We present protocols to create estrogen receptor positive (ER+) and androgen receptor positive (AR+) breast cancer models by combining organoid culture with mammary intraductal injection.

Keywords: Infection; Intraductal injection; Lentivirus; Transfection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Breast
Breast Neoplasms*
Female
Humans
Organoids
Receptors, Androgen

Substances

Receptors, Androgen