Does bacterial community succession within the polyethylene mulching film plastisphere drive biodegradation?

Abstract

Agricultural fields are severely contaminated with polyethylene mulching film (PMF) and this plastic in the natural environment can be colonized by biofilm-forming microorganisms that differ from those in the surrounding environment. In this study, we investigated the succession of the soil microbial communities in the PMF plastisphere using an artificial micro-ecosystem as well as exploring the degradation of PMF by plastisphere communities. The results indicated a significant and gradual decrease in the alpha diversity of the bacterial communities in the plastisphere and surrounding liquid. The community compositions in the plastisphere and surrounding liquid differed significantly from that in agricultural soil. Phyla and genera with the capacity to degrade polyethylene and hydrocarbon were enriched in the plastisphere, and some of these microorganisms were core members of the plastisphere community. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis detected increases in metabolism pathways for PMF plastisphere Xenobiotics Biodegradation and Metabolism, thereby suggesting the possibility of polyethylene degradation in the plastisphere. Observations by scanning electron microscopy (SEM) and confocal laser scanning microscopy demonstrated the formation of biofilms on the incubated PMF. SEM, atomic force microscopy, Fourier transform infrared spectroscopy and water contact angle detected significant changes in the surface microstructure, chemical composition and hydrophobicity change of the films, thereby suggesting that the plastisphere community degraded PMF during incubation. In conclusion, this study provides insights into the changes in agricultural soil microorganisms in the PMF plastisphere and the degradation of PMF.

Keywords: Biodegradation; Biofilm; Microbial succession; Plastisphere; Polyethylene mulching film.