Transcriptome regulation of extracellular vesicles derived from porcine uterine flushing fluids during peri-implantation on endometrial epithelial cells and embryonic trophoblast cells

Renwu Hua; Qiaorui Liu; Weisi Lian; Dengying Gao; Cheng Huang; Minggang Lei

doi:10.1016/j.gene.2022.146337

Transcriptome regulation of extracellular vesicles derived from porcine uterine flushing fluids during peri-implantation on endometrial epithelial cells and embryonic trophoblast cells

Gene. 2022 May 15:822:146337. doi: 10.1016/j.gene.2022.146337. Epub 2022 Feb 16.

Authors

Renwu Hua¹, Qiaorui Liu², Weisi Lian², Dengying Gao², Cheng Huang², Minggang Lei³

Affiliations

¹ Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education and Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430000, China; Shenzhen Key Laboratory of Fertility Regulation, Center of Assisted Reproduction and Embryology, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China.
² Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education and Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430000, China.
³ Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education and Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430000, China; National Engineering Research Center for Livestock, Wuhan 430000, China; The Cooperative Innovation Center for Sustainable Pig Production, 430000 Wuhan, China. Electronic address: leimg@mail.hzau.edu.cn.

PMID: 35182676
DOI: 10.1016/j.gene.2022.146337

Abstract

The extracellular vesicles (EVs) in uterine fluids play a vital role in embryo implantation by mediating intrauterine communication between conceptus and maternal endometrium in pigs. However, the regulatory mechanism of EVs in uterine fluids is largely unclear. In order to understand the effect of EVs in uterine flushing fluids (UFs) during embryo implantation on endometrial epithelial cells (EECs) and embryonic trophoblast cells (PTr2 cells). The UFs-EVs on day 13 of pregnancy (D13) were added to the culture medium of EECs and PTr2 cells. It was found that PKH-67 labeled UFs-EVs could be taken up in EECs and PTr2 cells. Transcriptome sequencing analysis showed that a total of 1793 and 6279 genes were differentially expressed in the EECs and PTr2 cells after the treatment of UFs-EVs on D13, respectively. Among these genes, real-time quantitative PCR (RT-qPCR) results indicated that ID2, ITGA5, CXCL10 and CXCL11 genes were differentially expressed in both EECs and PTr2 cells after treatment. Bioinformatics analysis showed that the differentially expressed (DE) genes in EECs and PTr2 cells after treatment are involved in immune regulation, cell migration, cell adhesion and the secretion and uptake of EVs. Our research offers novel insight into the regulation mechanism of UFs-EVs on D13 in EECs and PTr2 cells.

Keywords: Embryo implantation; Embryonic trophoblast cells; Endometrial epithelial cells; Extracellular vesicles; Uterine fluids.

MeSH terms

Animals
Cell Adhesion
Cell Culture Techniques
Cell Movement
Cells, Cultured
Embryo Implantation
Endometrium / cytology*
Endometrium / metabolism
Epithelial Cells / cytology
Epithelial Cells / metabolism
Extracellular Vesicles / transplantation*
Female
Gene Expression Profiling / veterinary*
Gene Expression Regulation
Gene Regulatory Networks*
Pregnancy
Sequence Analysis, RNA
Swine
Trophoblasts / cytology*
Trophoblasts / metabolism