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. 2022 Feb 21;7(1):56.
doi: 10.1038/s41392-022-00914-2.

Characterization of SARS-CoV-2 Omicron spike RBD reveals significantly decreased stability, severe evasion of neutralizing-antibody recognition but unaffected engagement by decoy ACE2 modified for enhanced RBD binding

Sheng Lin #  1 , Zimin Chen #  1 , Xindan Zhang  1 , Ao Wen  1 , Xin Yuan  1 , Chongzhang Yu  1 , Jing Yang  1 , Bin He  1 , Yu Cao  1   2 , Guangwen Lu  3
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Characterization of SARS-CoV-2 Omicron spike RBD reveals significantly decreased stability, severe evasion of neutralizing-antibody recognition but unaffected engagement by decoy ACE2 modified for enhanced RBD binding

Sheng Lin et al. Signal Transduct Target Ther. .
No abstract available

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Antibody-escape profile, receptor-binding capacity, and biochemical property of Omicron variant S-RBD. a Multiple previously identified antigenic sites were mapped on original strain S-RBD (PDB code: 6XC4). The RBS-A, RBS-B, RBS-C, RBS-D were circled on left panel, the CR3022 site and S309 site were circled on right panel. Amino-acid mutations of Omicron variant S-RBD were marked on both panels. b The binding affinities between SARS-CoV-2 S-RBD (original strain and Omicron variant) and each representative antibody (in scFv form) calculated by SPR. The dissociation constant (KD) values and associated affinity-fold decrease [KD (Omicron/Original] were individually shown. The antibodies that have been approved for clinical use were highlighted by shadowing in orange. The related real-time binding profiles were demonstrated in Supplementary Fig. S2. c–h The interaction between SARS-CoV-2 S-RBD (original strain and Omicron variant) and ACE2 proteins [wild-type or affinity-enhanced ACE2 mutants] characterized by SPR. The real-time binding profiles and calculated kinetic parameters are shown. i A DSF assay characterizing the thermostability of original strain and Omicron variant S-RBDs. The fluorescence-unit curve and melting temperature (Tm) for each S-RBD were shown. j, k Protease-digestion assays with fivefold serially diluted Trypsin (j) or Chymotrypsin (k) towards original strain S-RBD and Omicron variant S-RBD
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