Generation of three induced pluripotent stem cells lines from patients with esophageal atresia/tracheoesophageal fistula type C

Stem Cell Res. 2022 Apr;60:102711. doi: 10.1016/j.scr.2022.102711. Epub 2022 Feb 18.


Esophageal atresia/tracheoesophageal fistula (EA/TEF) is the most common congenital anomaly of the upper gastrointestinal tract affecting 1 in 3,000 which could stem from a developmental anomaly of the foregut. The cause is not fully understood. We generated three iPSC cell lines using peripheral blood mononuclear cells (PBMCs) from EA/TEF type C patients. Pluripotency and trilineage differentiation capacity of these three iPSC cell lines were confirmed by gene and protein expression profiles and the differentiation ability into the three germ layers. The generated disease-specific cell lines could serve as a tool to investigate the mechanisms of EA/TEF and acquired associated diseases.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Esophageal Atresia* / complications
  • Esophageal Atresia* / genetics
  • Humans
  • Induced Pluripotent Stem Cells*
  • Leukocytes, Mononuclear
  • Tracheoesophageal Fistula* / complications
  • Tracheoesophageal Fistula* / genetics

Supplementary concepts

  • Esophageal atresia with or without tracheoesophageal fistula