Lysozyme inhibits postharvest physiological deterioration of cassava

doi:10.1111/jipb.13219

. 2022 Mar;64(3):621-624.

doi: 10.1111/jipb.13219. Epub 2022 Feb 23.

Lysozyme inhibits postharvest physiological deterioration of cassava

Xiaoyun Wu^{1

2}, Jia Xu¹, Qiuxiang Ma¹, Sulaiman Ahmed¹, Xinlu Lu¹, Erjun Ling³, Peng Zhang^{1

2}

Affiliations

¹ National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, The Chinese Academy of Science, Shanghai, 200032, China.
² University of Chinese Academy of Sciences, Beijing, 100049, China.
³ CAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, The Chinese Academy of Science, Shanghai, 200032, China.

PMID: 35195347
DOI: 10.1111/jipb.13219

Lysozyme inhibits postharvest physiological deterioration of cassava

Xiaoyun Wu et al. J Integr Plant Biol. 2022 Mar.

. 2022 Mar;64(3):621-624.

doi: 10.1111/jipb.13219. Epub 2022 Feb 23.

Authors

Xiaoyun Wu^{1

2}, Jia Xu¹, Qiuxiang Ma¹, Sulaiman Ahmed¹, Xinlu Lu¹, Erjun Ling³, Peng Zhang^{1

2}

Affiliations

¹ National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, The Chinese Academy of Science, Shanghai, 200032, China.
² University of Chinese Academy of Sciences, Beijing, 100049, China.
³ CAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, The Chinese Academy of Science, Shanghai, 200032, China.

PMID: 35195347
DOI: 10.1111/jipb.13219

Abstract

After harvest, cassava (Manihot esculenta Crantz) storage roots undergo rapid postharvest physiological deterioration, producing blue-brown discoloration in the vasculature due to the production of polyphenolics (mainly quinones and coumarins) by enzymes such as polyphenol oxidase (PPO). Here, we report the application of hen egg-white lysozyme (HEWL), a natural PPO inhibitor, in transgenic cassava to repress the symptoms of postharvest physiological deterioration. The HEWL-expressing transgenic plants had lower levels of the two main cassava coumarins tested, scopoletin and scopolin, compared with wild type. HEWL-expressing cassava also showed increased tolerance of oxidative stress. Overall, the lysozyme-PPO system proved to be functional in plants for repressing PPO-mediated commercial product browning.

Keywords: cassava; inhibitor; lysozyme; polyphenol oxidase; post-harvest physiological deterioration.

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References

REFERENCES

1. Bayoumi, S.A., Rowan, M.G., Blagbrough, I.S., and Beeching, J.R. (2008). Biosynthesis of scopoletin and scopolin in cassava roots during post-harvest physiological deterioration: The E-Z-isomerisation stage. Phytochemistry 69: 2928-2936.
1. Düring, K., Porsch, P., Flaudung, M., and Lörz, H. (1993). Transgenic potato plants resistant to the phytopathogenic bacterium Erwinia carotovora. Plant J. 3: 587-598.
1. Goel, N., and Paul, P.K. (2015). Polyphenol oxidase and lysozyme mediate induction of systemic resistance in tomato, when a bioelicitor is used. J. Plant Protect. Res. 55: 343-350.
1. Nakajima, H., Muranaka, T., Ishige, F., Akutsu, K., and Oeda, K. (1997). Fungal and bacterial disease resistance in transgenic plants expressing human lysozyme. Plant Cell Rep. 16: 674-679.
1. Pei, X.W., Chen, S.K., Wen, R.M., Ye, S., Huang, J.Q., Zhang, Y.Q., Wang, B.S., Wang, J.X., and Jia, S.R. (2005). Creation of transgenic bananas expressing human lysozyme gene for Panama wilt resistance J. Integr. Plant Biol. 47: 971-977.

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[1] Bayoumi, S.A., Rowan, M.G., Blagbrough, I.S., and Beeching, J.R. (2008). Biosynthesis of scopoletin and scopolin in cassava roots during post-harvest physiological deterioration: The E-Z-isomerisation stage. Phytochemistry 69: 2928-2936.

[2] Bayoumi, S.A., Rowan, M.G., Blagbrough, I.S., and Beeching, J.R. (2008). Biosynthesis of scopoletin and scopolin in cassava roots during post-harvest physiological deterioration: The E-Z-isomerisation stage. Phytochemistry 69: 2928-2936.

[3] Düring, K., Porsch, P., Flaudung, M., and Lörz, H. (1993). Transgenic potato plants resistant to the phytopathogenic bacterium Erwinia carotovora. Plant J. 3: 587-598.

[4] Düring, K., Porsch, P., Flaudung, M., and Lörz, H. (1993). Transgenic potato plants resistant to the phytopathogenic bacterium Erwinia carotovora. Plant J. 3: 587-598.

[5] Goel, N., and Paul, P.K. (2015). Polyphenol oxidase and lysozyme mediate induction of systemic resistance in tomato, when a bioelicitor is used. J. Plant Protect. Res. 55: 343-350.

[6] Goel, N., and Paul, P.K. (2015). Polyphenol oxidase and lysozyme mediate induction of systemic resistance in tomato, when a bioelicitor is used. J. Plant Protect. Res. 55: 343-350.

[7] Nakajima, H., Muranaka, T., Ishige, F., Akutsu, K., and Oeda, K. (1997). Fungal and bacterial disease resistance in transgenic plants expressing human lysozyme. Plant Cell Rep. 16: 674-679.

[8] Nakajima, H., Muranaka, T., Ishige, F., Akutsu, K., and Oeda, K. (1997). Fungal and bacterial disease resistance in transgenic plants expressing human lysozyme. Plant Cell Rep. 16: 674-679.

[9] Pei, X.W., Chen, S.K., Wen, R.M., Ye, S., Huang, J.Q., Zhang, Y.Q., Wang, B.S., Wang, J.X., and Jia, S.R. (2005). Creation of transgenic bananas expressing human lysozyme gene for Panama wilt resistance J. Integr. Plant Biol. 47: 971-977.

[10] Pei, X.W., Chen, S.K., Wen, R.M., Ye, S., Huang, J.Q., Zhang, Y.Q., Wang, B.S., Wang, J.X., and Jia, S.R. (2005). Creation of transgenic bananas expressing human lysozyme gene for Panama wilt resistance J. Integr. Plant Biol. 47: 971-977.

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Lysozyme inhibits postharvest physiological deterioration of cassava

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Lysozyme inhibits postharvest physiological deterioration of cassava

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