Astrocyte-neuron crosstalk through Hedgehog signaling mediates cortical synapse development

Cell Rep. 2022 Feb 22;38(8):110416. doi: 10.1016/j.celrep.2022.110416.


Neuron-glia interactions play a critical role in the regulation of synapse formation and circuit assembly. Here we demonstrate that canonical Sonic hedgehog (Shh) pathway signaling in cortical astrocytes acts to coordinate layer-specific synaptic connectivity. We show that the Shh receptor Ptch1 is expressed by cortical astrocytes during development and that Shh signaling is necessary and sufficient to promote the expression of genes involved in regulating synaptic development and layer-enriched astrocyte molecular identity. Loss of Shh in layer V neurons reduces astrocyte complexity and coverage by astrocytic processes in tripartite synapses; conversely, cell-autonomous activation of Shh signaling in astrocytes promotes cortical excitatory synapse formation. Furthermore, Shh-dependent genes Lrig1 and Sparc distinctively contribute to astrocyte morphology and synapse formation. Together, these results suggest that Shh secreted from deep-layer cortical neurons acts to specialize the molecular and functional features of astrocytes during development to shape circuit assembly and function.

Keywords: Lrig1; Sonic hedgehog; Sparc; astrocytes; cortical circuits; neuron-glia interaction; synapse formation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Astrocytes* / metabolism
  • Hedgehog Proteins* / metabolism
  • Neurogenesis / physiology
  • Neurons / metabolism
  • Synapses / metabolism


  • Hedgehog Proteins