A protocol for full-rotation soft X-ray tomography of single cells

STAR Protoc. 2022 Feb 10;3(1):101176. doi: 10.1016/j.xpro.2022.101176. eCollection 2022 Mar 18.


The protocol describes step-by-step sample preparation, data acquisition, and segmentation of cellular organelles with soft X-ray tomography. It is designed for microscopes built to perform full-rotation data acquisition on specimens in cylindrical sample holders, such as the XM-2 microscope at the Advanced Light Source, LBNL; however, it might be generalized for similar sample holder designs for both synchrotron and table-top microscopes. For complete details on the use and execution of this profile, please refer to Loconte et al. (2021).

Keywords: Cell Biology; Microscopy; Single Cell.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Imaging, Three-Dimensional* / methods
  • Microscopy / methods
  • Rotation
  • Synchrotrons
  • Tomography, X-Ray* / methods