Mismatch binding ligand upregulated back-splicing reaction producing circular RNA in a cellular model

Chem Commun (Camb). 2022 Mar 15;58(22):3629-3632. doi: 10.1039/d1cc06936e.


Circular RNA (circRNA) is a covalently closed single-stranded RNA produced from pre-mRNAs via back-splicing reaction, an alternative form of splicing. Here, we show naphthyridine carbamate dimer (NCD) upregulating the production of a circRNA from a pre-mRNA containing NCD-binding site UGGAA/UGGAA in cells, demonstrating the feasibility of small-molecule mediated circRNA production.

MeSH terms

  • Ligands
  • RNA / genetics
  • RNA / metabolism
  • RNA Precursors / genetics
  • RNA Splicing*
  • RNA, Circular*


  • Ligands
  • RNA Precursors
  • RNA, Circular
  • RNA