Delivery of Antisense Oligonucleotides to the Mouse Brain by Intracerebroventricular Injections

Tom Metz; Elsa C Kuijper; Willeke M C van Roon-Mom

doi:10.1007/978-1-0716-2010-6_23

Delivery of Antisense Oligonucleotides to the Mouse Brain by Intracerebroventricular Injections

Methods Mol Biol. 2022:2434:333-341. doi: 10.1007/978-1-0716-2010-6_23.

Authors

Tom Metz¹, Elsa C Kuijper², Willeke M C van Roon-Mom²

Affiliations

¹ Department of Human Genetics, LUMC, Leiden, The Netherlands. t.metz@lumc.nl.
² Department of Human Genetics, LUMC, Leiden, The Netherlands.

Abstract

The use of antisense oligonucleotides (AONs) is a promising therapeutic strategy for central nervous system disorders. However, the delivery of AONs to the central nervous system is challenging because their size does not allow them to diffuse over the blood-brain barrier (BBB) when injected systemically. The BBB can be bypassed by administering directly into the brain. Here we describe a method to perform single and repeated intracerebroventricular injections into the lateral ventricle of the mouse brain.

Keywords: Antisense oligonucleotides; Cannula; Intracerebroventricular injections; Stereotactic surgery.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blood-Brain Barrier
Brain
Central Nervous System
Central Nervous System Diseases*
Injections, Intraventricular
Mice
Oligonucleotides, Antisense* / genetics

Substances

Oligonucleotides, Antisense