Diphyllin Shows a Broad-Spectrum Antiviral Activity against Multiple Medically Important Enveloped RNA and DNA Viruses

Viruses. 2022 Feb 9;14(2):354. doi: 10.3390/v14020354.


Diphyllin is a natural arylnaphtalide lignan extracted from tropical plants of particular importance in traditional Chinese medicine. This compound has been described as a potent inhibitor of vacuolar (H+)ATPases and hence of the endosomal acidification process that is required by numerous enveloped viruses to trigger their respective viral infection cascades after entering host cells by receptor-mediated endocytosis. Accordingly, we report here a revised, updated, and improved synthesis of diphyllin, and demonstrate its antiviral activities against a panel of enveloped viruses from Flaviviridae, Phenuiviridae, Rhabdoviridae, and Herpesviridae families. Diphyllin is not cytotoxic for Vero and BHK-21 cells up to 100 µM and exerts a sub-micromolar or low-micromolar antiviral activity against tick-borne encephalitis virus, West Nile virus, Zika virus, Rift Valley fever virus, rabies virus, and herpes-simplex virus type 1. Our study shows that diphyllin is a broad-spectrum host cell-targeting antiviral agent that blocks the replication of multiple phylogenetically unrelated enveloped RNA and DNA viruses. In support of this, we also demonstrate that diphyllin is more than just a vacuolar (H+)ATPase inhibitor but may employ other antiviral mechanisms of action to inhibit the replication cycles of those viruses that do not enter host cells by endocytosis followed by low pH-dependent membrane fusion.

Keywords: antiviral activity; cleistanthin B; cytotoxicity; diphyllin; enveloped virus; vacuolar ATPase inhibitor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Viral / metabolism
  • Antiviral Agents / chemical synthesis
  • Antiviral Agents / pharmacology*
  • Cell Line
  • Cell Survival / drug effects
  • Glucosides / pharmacology
  • Lignans / chemical synthesis
  • Lignans / pharmacology*
  • Vacuolar Proton-Translocating ATPases / antagonists & inhibitors
  • Virus Replication / drug effects
  • Viruses / classification
  • Viruses / drug effects*
  • Viruses / metabolism


  • Antigens, Viral
  • Antiviral Agents
  • Glucosides
  • Lignans
  • cleistanthin B
  • Vacuolar Proton-Translocating ATPases
  • diphyllin