The PMMA/PVC/ZnO-nanocomposites with zinc oxide nanoparticle (particle size < 50nm) was synthesized by solution casting technique. Morphology of the synthesized nano composites have been investigated by FT-IR and XRD techniques. After characterization, synthesized composites were applied for antibacterial, selective antibiofilm and free radical scavenging screening. Antibacterial studies were measured against different bacterial strains. Antibiofilms activities were studied against those bacterial model pathogenic strains which showed highest and minimum sensitivity as a (~94 and ~88 at 160 μg/ml). Antioxidant activity of synthesized nanocomposites were measured by DPPH and showed scavenging capacity with IC50, 110 to > 200 μg/mL. Thus PMMA/PVC/ZnO nanocomposite showed promising antimicrobial activity and antioxidant activity that can be used for biomedical applications.