Isolation of neurons suitable for patch-clamping from adult mammalian central nervous systems

J Neurosci Methods. 1986 May;16(3):227-38. doi: 10.1016/0165-0270(86)90040-3.


A method is described for the isolation of neurons from defined regions of the mammalian central nervous system, by a combination of mechanical and enzymatic means. The procedure liberates neurons free of cellular debris and glial investments, allowing the formation of giga-ohm seals with patch clamp electrodes. The characteristic morphology of neurons is maintained, together with the diversity of active channels evident in the intact nervous system.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aging
  • Animals
  • Brain / cytology*
  • Cell Separation / methods*
  • Cell Survival
  • Electric Stimulation
  • Guinea Pigs
  • Membrane Potentials
  • Microscopy, Electron, Scanning
  • Microscopy, Phase-Contrast
  • Neurons / physiology*
  • Neurons / ultrastructure