Generation of an induced pluripotent stem cell line from a healthy Caucasian male

Stem Cell Res. 2022 Apr;60:102717. doi: 10.1016/j.scr.2022.102717. Epub 2022 Feb 22.


The effects of genetic mutations on protein function can be studied in a physiologically relevant environment using tissue-specific cells differentiated from patient-derived induced pluripotent stem cells (iPSC). However, it is crucial to use iPSC derived from healthy individuals as control. We generated an iPS cell line from skin fibroblasts of a healthy Caucasian male by nucleofection of non-integrating episomal vectors. This cell line has normal karyotype, expresses pluripotency surface markers and pluripotency genes, and successfully differentiates into cells of the 3 germ layers. Therefore, it can be used as control for any disease of interest that is modelled using iPSC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation
  • Cell Line
  • Fibroblasts
  • Germ Layers
  • Humans
  • Induced Pluripotent Stem Cells* / metabolism
  • Male