Abstract
A goat anti-serum was prepared against mucinous ovarian cyst fluid and absorbed with normal colon and a variety of normal tissues until the only residual immunoreactivity was directed against colon cancer and ovarian tumor mucin. The set of antigenic determinants defined by this anti-serum has been called COTA, standing for colon-ovarian-tumor-antigen. This highly absorbed anti-serum (anti-COTA) was used for immunohistochemical staining of 42 different tissues in parallel with staining with a goat anti-CEA, which was also highly absorbed. The results suggest that COTA is a highly sensitive and specific antigen for colon carcinoma and may have potential for the early detection of malignant changes predictive of cancer of the colon.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenocarcinoma / diagnosis
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Adenocarcinoma / immunology
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Adenocarcinoma / pathology
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Adenoma / diagnosis
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Adenoma / immunology
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Adenoma / pathology
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Animals
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Antibody Specificity
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Antigens, Neoplasm / analysis*
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Antigens, Neoplasm / immunology
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Carcinoembryonic Antigen / analysis*
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Carcinoembryonic Antigen / immunology
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Colitis, Ulcerative / diagnosis
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Colitis, Ulcerative / immunology
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Colitis, Ulcerative / pathology
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Colon / immunology
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Colon / pathology
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Colonic Neoplasms / diagnosis*
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Colonic Neoplasms / immunology
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Colonic Neoplasms / pathology
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Diagnosis, Differential
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Female
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Humans
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Immunoenzyme Techniques
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Neoplasm Transplantation
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Ovarian Neoplasms / diagnosis*
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Ovarian Neoplasms / immunology
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Ovarian Neoplasms / pathology
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Rats
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Rats, Inbred Strains
Substances
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Antigens, Neoplasm
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Carcinoembryonic Antigen
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colon-ovarian tumor antigen, human