Flow-cytometry-based protocol to analyze respiratory chain function in mouse microglia

STAR Protoc. 2022 Feb 18;3(1):101186. doi: 10.1016/j.xpro.2022.101186. eCollection 2022 Mar 18.


Most of the protocols to analyze metabolic features of cell populations from different tissues rely on in vitro cell culture conditions. Here, we present a flow-cytometry-based protocol for measuring the respiratory chain function in permeabilized mouse microglia ex vivo. We describe microglial cell isolation, followed by analyzing complex I and II using flow cytometry. This optimized protocol requires a low input of permeabilized cells and can be applied to other ex vivo isolated cells or cells derived from cell cultures. For complete details on the use and execution of this protocol, please refer to Erny et al. (2021).

Keywords: Cell Biology; Cell isolation; Flow Cytometry/Mass Cytometry; Immunology; Metabolism; Neuroscience.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques*
  • Cell Separation / methods
  • Electron Transport
  • Flow Cytometry / methods
  • Mice
  • Microglia*