Composite alginate-gelatin hydrogels incorporating PRGF enhance human dental pulp cell adhesion, chemotaxis and proliferation

Int J Pharm. 2022 Apr 5:617:121631. doi: 10.1016/j.ijpharm.2022.121631. Epub 2022 Mar 2.


The increasing prevalence of tissue injuries is fueling the development of autologous biological treatments for regenerative medicine. Here, we investigated the potential of three different bioinks based on the combination of gelatin and alginate (GA), enriched in either hydroxyapatite (GAHA) or hydroxyapatite and PRGF (GAHAP), as a favorable microenvironment for human dental pulp stem cells (DPSCs). Swelling behaviour, in vitro degradation and mechanical properties of the matrices were evaluated. Morphological and elemental analysis of the scaffolds were also performed along with cytocompatibility studies. The in vitro cell response to the different scaffolds was also assessed. Results showed that all scaffolds presented high swelling capacity, and those that contained HA showed higher Young's modulus. GAHAP had the lowest degradation rate and the highest values of cytocompatibility. Cell adhesion and chemotaxis were significantly increased when PRGF was incorporated to the matrices. GAHA and GAHAP compositions promoted the highest proliferative rate as well as significantly stimulated osteogenic differentiation. In conclusion, the enrichment with PRGF improves the regenerative properties of the composites favouring the development of personalized constructs.

Keywords: Bone; Drug delivery; Gelatin; PRGF; Scaffold; Tissue engineering.

MeSH terms

  • Alginates*
  • Cell Adhesion
  • Cell Differentiation
  • Cell Proliferation
  • Chemotaxis
  • Dental Pulp
  • Gelatin*
  • Humans
  • Hydrogels
  • Osteogenesis
  • Tissue Engineering / methods
  • Tissue Scaffolds


  • Alginates
  • Hydrogels
  • Gelatin