The Migration and Neurochemical Differentiation of Gamma-Aminobutyric Acid (GABA)-immunoreactive Neurons in Rat Visual Cortex as Demonstrated by a Combined Immunocytochemical-Autoradiographic Technique

Brain Res. 1986 Jul;393(1):41-6. doi: 10.1016/0165-3806(86)90063-5.

Abstract

The development of some cortical local circuit neurons was traced using a technique which combined immunocytochemistry to label gamma-aminobutyric acid-immunoreactive (GABA+) neurons and [3H]thymidine autoradiography to identify cells with similar times of origin. Double-labeled neurons (GABA+ neurons with autoradiographic silver grains over their nuclei) are evident only after migration is complete. GABA+ neurons born on gestational day 16 are first identified in layer VIa on postnatal day 0 and those born on day 19 are in layer III on postnatal day 6. The frequency of double-labeled neurons increases during the week following their appearance. Therefore, the migration and the neurochemical differentiation of GABA+ neurons follow inside-to-outside sequences.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Autoradiography
  • Cell Differentiation
  • Cell Movement
  • Immunoenzyme Techniques
  • Rats
  • Visual Cortex / cytology
  • Visual Cortex / growth & development*
  • Visual Cortex / metabolism
  • gamma-Aminobutyric Acid / metabolism*

Substances

  • gamma-Aminobutyric Acid