A new network containing MYB109-ZmCesA5 is involved in kernel development

Erfei Dong; Chaoxian Liu; Yang Bai; Xiupeng Mei; Zikun Zhao; Siying Zhu; Jiahao Zhang; Yilin Cai

doi:10.1016/j.bbrc.2022.02.104

A new network containing MYB109-ZmCesA5 is involved in kernel development

Biochem Biophys Res Commun. 2022 Apr 30:602:15-20. doi: 10.1016/j.bbrc.2022.02.104. Epub 2022 Feb 28.

Authors

Erfei Dong¹, Chaoxian Liu¹, Yang Bai¹, Xiupeng Mei¹, Zikun Zhao¹, Siying Zhu¹, Jiahao Zhang¹, Yilin Cai²

Affiliations

¹ Maize Research Institute, Southwest University, Tiansheng Road, No. 2, Beibei District, Chongqing, China.
² Maize Research Institute, Southwest University, Tiansheng Road, No. 2, Beibei District, Chongqing, China. Electronic address: caiyilin1789@163.com.

PMID: 35247699
DOI: 10.1016/j.bbrc.2022.02.104

Abstract

MYB genes regulate several different aspects of metabolism and development. However, few studies have reported the involvement of MYBs-CesAs network in the regulation of maize kernel development. In this study, yeast one-hybrid (Y1H) assays and dual-luciferase reporter assays showed that ZmMYB109 activated the expression of ZmCesA5 by directly binding to its promoter. Real-time quantitative PCR (RT-qPCR) and transcriptome analyses showed that ZmMYB109 expression increased in ZmCesA5-OE kernels and decreased in ZmCesA5-KO kernels. Overexpression of ZmCesA5 produced heavier kernels, whereas loss of function of ZmCesA5 affected starch and sucrose metabolism, resulting in weight reduction of the maize kernels. Collectively, these findings suggest that a new network containing MYB109-ZmCesA5 is involved in kernel development.

Keywords: Kernel development; Maize; ZmCesA5; ZmMYB109.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Metabolism
Gene Expression Profiling
Starch* / metabolism
Zea mays* / metabolism

Substances

Starch