Identification of a protein-binding site that mediates transcriptional response of the c-fos gene to serum factors

Cell. 1986 Aug 15;46(4):567-74. doi: 10.1016/0092-8674(86)90882-2.


Transient transcriptional activation of the c-fos gene following serum stimulation of susceptible cells requires a conserved DNA element located 300 bp 5' to the mRNA cap site. A DNA-binding gel electrophoresis assay was used to detect a protein(s) in HeLa cell nuclear extracts that specifically binds to the 5' activating element. The protein recognizes a region of dyad symmetry within the 5' activating element, defined by binding competition, dimethylsulphate (DMS) interference and DNAase I and DMS protection studies. A single 22 bp synthetic copy of the dyad symmetry element will both compete efficiently for protein binding and restore serum regulation to c-fosH genes that lack the 5' activating element.

MeSH terms

  • Base Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics
  • Deoxyribonuclease I / metabolism
  • Enhancer Elements, Genetic*
  • Female
  • Gene Expression Regulation
  • Genes
  • Genes, Regulator*
  • HeLa Cells
  • Methylation
  • Protein Binding
  • Proto-Oncogene Proteins / genetics*
  • Transcription Factors / genetics*
  • Transcription, Genetic


  • DNA-Binding Proteins
  • Proto-Oncogene Proteins
  • Transcription Factors
  • Deoxyribonuclease I