Production and characterization of bioactive peptides from rice beans using Bacillus subtilis

Bioresour Technol. 2022 May;351:126932. doi: 10.1016/j.biortech.2022.126932. Epub 2022 Mar 3.


A bioprocess was developed for production of bioactive peptides on microbial fermentation of rice beans using proteolytic Bacillus subtilis strains. The peptides produced were identified by LC-MS/MS analysis, revealing the presence of many unique peptide sequences to individual hydrolysates. On functional properties prediction, antihypertensive peptides (3.90%) were found to be higher in comparison to other bioactive peptides. Among different strains, B. subtilis KN2B fermented hydrolysate exhibited highest angiotensin converting enzyme (ACE)-inhibitory activity (45.73%). Furthermore, 19 selected peptides, including the common and unique peptides were examined for their affinity towards the binding cavity of ACE using molecular docking. The results showed a common peptide PFPIPFPIPIPLP, and another IPFPPIPFLPPI unique to B. subtilis KN2B fermented hydrolysate exhibited promising binding at the ACE binding site with substantial free binding energy. The process developed can be used for the production of bioactive peptides from rice bean for application in nutraceutical industries.

Keywords: ACE-inhibitory peptide; Bacillus subtilis; Fermented legume; Molecular docking; Rice bean.

MeSH terms

  • Angiotensin-Converting Enzyme Inhibitors / chemistry
  • Angiotensin-Converting Enzyme Inhibitors / metabolism
  • Angiotensin-Converting Enzyme Inhibitors / pharmacology
  • Bacillus subtilis* / metabolism
  • Chromatography, Liquid
  • Molecular Docking Simulation
  • Peptides / metabolism
  • Tandem Mass Spectrometry
  • Vigna* / metabolism


  • Angiotensin-Converting Enzyme Inhibitors
  • Peptides