Protective Roles of Shilajit in Modulating Resistin, Adiponectin, and Cytokines in Rats with Non-alcoholic Fatty Liver Disease

Chin J Integr Med. 2022 Jun;28(6):531-537. doi: 10.1007/s11655-022-3307-3. Epub 2022 Mar 8.

Abstract

Objective: To evaluate the effect of Shilajit, a medicine of Ayurveda, on the serum changes in cytokines and adipokines caused by non-alcoholic fatty liver disease (NAFLD).

Methods: After establishing fatty liver models by feeding a high-fat diet (HFD) for 12 weeks, 35 Wistar male rats were randomly divided into 5 groups, including control (standard diet), Veh (HFD + vehicle), high-dose Shilajit [H-Sh, HFD + 250 mg/(kg·d) Shilajit], low-dose Shilajit [L-Sh, HFD + 150 mg/(kg·d) Shilajit], and pioglitazone [HFD + 10 mg/(kg·d) pioglitazone] groups, 7 rats in each group. After 2-week of gavage administration, serum levels of glucose, insulin, interleukin 1beta (IL-1β), IL-6, IL-10, tumor necrosis factor-alpha (TNF-α), adiponectin, and resistin were measured, and insulin resistance index (HOMA-IR) was calculated.

Results: After NAFLD induction, the serum level of IL-10 significantly increased and serum IL-1β, TNF-α levels significantly decreased by injection of both doses of Shilajit and pioglitazone (P<0.05). Increases in serum glucose level and homeostasis model of HOMA-IR were reduced by L-Sh and H-Sh treatment in NAFLD rats (P<0.05). Both doses of Shilajit increased adiponectin and decreased serum resistin levels (P<0.05).

Conclusion: The probable protective role of Shilajit in NAFLD model rats may be via modulating the serum levels of IL-1β, TNF-α, IL-10, adipokine and resistin, and reducing of HOMA-IR.

Keywords: Ayurvedic medicine; Shilajit; adipokines; cytokines; dietary supplement; glucose; insulin; non-alcoholic fatty liver disease.

MeSH terms

  • Adiponectin
  • Animals
  • Cytokines
  • Diet, High-Fat
  • Glucose
  • Insulin Resistance*
  • Interleukin-10
  • Liver
  • Male
  • Minerals
  • Non-alcoholic Fatty Liver Disease* / drug therapy
  • Non-alcoholic Fatty Liver Disease* / pathology
  • Pioglitazone / pharmacology
  • Pioglitazone / therapeutic use
  • Rats
  • Rats, Wistar
  • Resins, Plant
  • Resistin / pharmacology
  • Resistin / therapeutic use
  • Tumor Necrosis Factor-alpha

Substances

  • Adiponectin
  • Cytokines
  • Minerals
  • Resins, Plant
  • Resistin
  • Tumor Necrosis Factor-alpha
  • mumie
  • Interleukin-10
  • Glucose
  • Pioglitazone