Analysis of the pheromone signaling pathway by RT-qPCR in the budding yeast Saccharomyces cerevisiae

STAR Protoc. 2022 Mar 3;3(1):101210. doi: 10.1016/j.xpro.2022.101210. eCollection 2022 Mar 18.


FUS3 and STE2 expression levels can be used as reporters for signaling through the pheromone pathway in the budding yeast Saccharomyces cerevisiae. Here, we describe an optimized protocol to measure the expression levels of FUS3 and STE2 using quantitative reverse transcription PCR (RT-qPCR). We describe the steps for comparing untreated and pheromone-treated yeast cells and how to quantify the changes in various deletion strains. The protocol can be applied to determine potential regulators of the pheromone pathway. For complete details on the use and execution of this protocol, please refer to Garcia et al. (2021).

Keywords: Cell Biology; Gene Expression; Model Organisms; Molecular Biology; Signal Transduction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Mitogen-Activated Protein Kinases / metabolism
  • Pheromones / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins* / genetics
  • Signal Transduction / genetics
  • Yeast, Dried*


  • Pheromones
  • Saccharomyces cerevisiae Proteins
  • FUS3 protein, S cerevisiae
  • Mitogen-Activated Protein Kinases