Single-cell technologies to decipher cardiovascular diseases

Eur Heart J. 2022 Mar 10;ehac095. doi: 10.1093/eurheartj/ehac095. Online ahead of print.

Abstract

Cardiovascular disease remains the leading cause of death worldwide. A deeper understanding of the multicellular composition and molecular processes may help to identify novel therapeutic strategies. Single-cell technologies such as single-cell or single-nuclei RNA sequencing provide expression profiles of individual cells and allow for dissection of heterogeneity in tissue during health and disease. This review will summarize (i) how these novel technologies have become critical for delineating mechanistic drivers of cardiovascular disease, particularly, in humans and (ii) how they might serve as diagnostic tools for risk stratification or individualized therapy. The review will further discuss technical pitfalls and provide an overview of publicly available human and mouse data sets that can be used as a resource for research.

Keywords: Atherosclerosis; Diagnostic; Heart failure; Hypertrophy; Single-cell sequencing; Single-nuclei sequencing.