Phosphoinositide 3 Kinase γ Plays a Critical Role in Acute Kidney Injury

Cells. 2022 Feb 23;11(5):772. doi: 10.3390/cells11050772.


Inflammatory cells contribute to the pathogenesis of renal ischemia-reperfusion injury (IRI). However, the signaling mechanisms underlying the infiltration of inflammatory cells into the kidney are not well understood. In this study, we examined the effects of phosphoinositide 3 kinase γ (PI3Kγ) on inflammatory cells infiltration into the kidney in response to ischemia-reperfusion injury. Compared with wild-type mice, PI3Kγ knockout mice displayed less IRI in the kidney with fewer tubular apoptotic cell. Furthermore, PI3Kγ deficiency decreased the number of infiltrated neutrophils, macrophages, and T cells in the kidney, which was accompanied by a decrease in the expression of pro-inflammatory cytokines in the kidney. Moreover, wild-type mice treated with AS-605240, a selective PI3Kγ inhibitor, displayed less tubular damage, accumulated fewer inflammatory cells, and expressed less proinflammatory molecules in the kidney following IRI. These results demonstrate that PI3Kγ has a critical role in the pathogenesis of kidney damage in IRI, indicating that PI3Kγ inhibition may serve as a potential therapeutic strategy for the prevention of ischemia-reperfusion-induced kidney injury.

Keywords: PI3Kγ; acute kidney injury; inflammation; ischemia-reperfusion injury.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Kidney Injury* / metabolism
  • Animals
  • Class Ib Phosphatidylinositol 3-Kinase / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Phosphatidylinositol 3-Kinase
  • Phosphatidylinositol 3-Kinases
  • Reperfusion Injury* / metabolism


  • Class Ib Phosphatidylinositol 3-Kinase
  • Phosphatidylinositol 3-Kinase
  • Pik3cg protein, mouse