CRISPR/Cas9-engineered human ES cells harboring heterozygous and homozygous c-KIT knockout

Stem Cell Res. 2022 Apr:60:102732. doi: 10.1016/j.scr.2022.102732. Epub 2022 Mar 1.


The receptor tyrosine kinase c-KIT (CD117) has a key role in hematopoiesis and is a marker for endothelial and cardiac progenitor cells. In vivo, deficiency of c-KIT is lethal and therefore using CRISPR/Cas9 editing we generated heterozygous and homozygous c-KIT knockout human embryonic stem cell (ES cell) lines. The c-KIT knockout left ES cell pluripotency unaffected as shown by immunofluorescence and trilineage differentiation potential. Heterozygous and homozygous c-KIT knockouts showed complete loss of exon 17, resulting in ablation of c-KIT protein from the cell surface. c-KIT knockout ES cells provide a valuable tool for further investigating c-KIT biology.

Keywords: CD117; CRISPR; ES cells; Embryonic stem cells; Stem cell factor receptor; c-KIT.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CRISPR-Cas Systems / genetics
  • Cell Line
  • Heterozygote
  • Homozygote
  • Human Embryonic Stem Cells* / metabolism
  • Humans