Relaxation optimized double acquisition (RODA) as an alternative for virtual decoupling of NMR spectra

Alvar D Gossert; Gerhard Wider

doi:10.1016/j.jmr.2022.107177

Relaxation optimized double acquisition (RODA) as an alternative for virtual decoupling of NMR spectra

J Magn Reson. 2022 Apr:337:107177. doi: 10.1016/j.jmr.2022.107177. Epub 2022 Mar 4.

Authors

Alvar D Gossert¹, Gerhard Wider²

Affiliations

¹ Department of Biology, Biomolecular NMR Spectroscopy Platform, ETH Zürich, 8093 Zürich, Switzerland. Electronic address: alvar.gossert@biol.ethz.ch.
² Department of Biology, Biomolecular NMR Spectroscopy Platform, ETH Zürich, 8093 Zürich, Switzerland.

PMID: 35290935
DOI: 10.1016/j.jmr.2022.107177

Abstract

We introduce an alternative way for spin-state selection, RODA, which yields higher sensitivity for spin systems exhibiting a TROSY effect. With RODA, the TROSY component of a doublet is recorded twice using a double acquisition scheme. RODA works by simple addition of consecutive NMR signals, and does not require any special processing. Thus, this pulse sequence element can seamlessly be integrated into existing experiments. We demonstrate the broad applicability of RODA with several systems exhibiting a TROSY effect on ¹⁵N-¹H, ¹⁹F-¹³C or ¹H-¹³C moieties. Further, we show that virtual decoupling with increased sensitivity is possible in a single double acquisition experiment in situations as encountered with dissolution DNP.

Keywords: Dissolution DNP; Double acquisition; IPAP; Spin-state selection; TROSY; Virtual decoupling.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Magnetic Resonance Imaging*
Magnetic Resonance Spectroscopy
Nuclear Magnetic Resonance, Biomolecular