Detection techniques for epitranscriptomic marks

Am J Physiol Cell Physiol. 2022 Apr 1;322(4):C787-C793. doi: 10.1152/ajpcell.00460.2021. Epub 2022 Mar 16.


Similar to epigenetic DNA modification, RNA can be methylated and altered for stability and processing. RNA modifications, namely, epitranscriptomes, involve the following three functions: writing, erasing, and reading of marks. Methods for measurement and position detection are useful for the assessment of cellular function and human disease biomarkers. After pyrimidine 5-methylcytosine was reported for the first time a hundred years ago, numerous techniques have been developed for studying nucleotide modifications, including RNAs. Recent studies have focused on high-throughput and direct measurements for investigating the precise function of epitranscriptomes, including the characterization of severe acute respiratory syndrome coronavirus 2. The current study presents an overview of the development of detection techniques for epitranscriptomic marks and briefs about the recent progress in this field.

Keywords: RNA; epitranscriptomics; methylation; techniques; virus.

Publication types

  • Review
  • Research Support, Non-U.S. Gov't

MeSH terms

  • COVID-19*
  • Epigenesis, Genetic
  • Humans
  • RNA / genetics
  • RNA / metabolism
  • RNA Processing, Post-Transcriptional
  • Transcriptome* / genetics


  • RNA