Compartment-specific tuning of dendritic feature selectivity by intracellular Ca2+ release

Science. 2022 Mar 18;375(6586):eabm1670. doi: 10.1126/science.abm1670. Epub 2022 Mar 18.


Dendritic calcium signaling is central to neural plasticity mechanisms that allow animals to adapt to the environment. Intracellular calcium release (ICR) from the endoplasmic reticulum has long been thought to shape these mechanisms. However, ICR has not been investigated in mammalian neurons in vivo. We combined electroporation of single CA1 pyramidal neurons, simultaneous imaging of dendritic and somatic activity during spatial navigation, optogenetic place field induction, and acute genetic augmentation of ICR cytosolic impact to reveal that ICR supports the establishment of dendritic feature selectivity and shapes integrative properties determining output-level receptive fields. This role for ICR was more prominent in apical than in basal dendrites. Thus, ICR cooperates with circuit-level architecture in vivo to promote the emergence of behaviorally relevant plasticity in a compartment-specific manner.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials
  • Adaptor Proteins, Signal Transducing / genetics
  • Animals
  • CA1 Region, Hippocampal / physiology*
  • Calcium / metabolism*
  • Calcium Signaling
  • Cytosol / metabolism
  • Dendrites / physiology*
  • Electroporation
  • Endoplasmic Reticulum / metabolism*
  • Female
  • Male
  • Mice
  • Neuronal Plasticity*
  • Optogenetics
  • Place Cells / physiology*
  • Single-Cell Analysis
  • Spatial Navigation


  • Adaptor Proteins, Signal Transducing
  • Pdzd8 protein, mouse
  • Calcium