Glucagon-like Peptides GLP-1 and GLP-2, Predicted Products of the Glucagon Gene, Are Secreted Separately From Pig Small Intestine but Not Pancreas

Endocrinology. 1986 Oct;119(4):1467-75. doi: 10.1210/endo-119-4-1467.

Abstract

We developed specific antibodies and RIAs for glucagon-like peptides 1 and 2 (GLP-1 and GLP-2), two predicted products of the glucagon gene, and studied the occurrence, nature, and secretion of immunoreactive GLP-1 and GLP-2 in pig pancreas and small intestine. Immunoreactive GLP-1 and GLP-2 were identified in glucagon-producing cells of the pancreatic islets, and in glicentin-producing cells of the small intestine. Immunoreactive GLP-1 and 2 in intestinal extracts corresponded in molecular size to peptides synthesized according to the predicted structure. By reverse phase HPLC, intestinal and synthetic GLP-1 behaved similarly, whereas synthetic and intestinal GLP-2 differed. Pancreatic extracts contained a large peptide with both GLP-1 and GLP-2 immunoreactivity. Secretion was studied using isolated perfused pig pancreas during arginine stimulation, and isolated perfused pig ileum during either luminal glucose stimulation or vascular administration of the neuropeptide, gastrin-releasing peptide (GRP). Immunoreactive GLP-1 and GLP-2 were secreted in parallel with pancreatic glucagon and intestinal glicentin. The molecular forms of secreted immunoreactive GLP-1 and 2 corresponded to those identified in the tissue extracts.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arginine / pharmacology
  • Gastrin-Releasing Peptide
  • Glucagon / metabolism
  • Glucagon-Like Peptide 1
  • Glucagon-Like Peptide 2
  • Glucose / pharmacology
  • Histocytochemistry
  • Immunoenzyme Techniques
  • Intestine, Small / analysis
  • Intestine, Small / drug effects
  • Intestine, Small / metabolism*
  • Pancreas / analysis
  • Pancreas / drug effects
  • Pancreas / metabolism*
  • Peptides / analysis
  • Peptides / metabolism*
  • Peptides / pharmacology
  • Proglucagon
  • Protein Precursors / metabolism
  • Swine

Substances

  • Glucagon-Like Peptide 2
  • Peptides
  • Protein Precursors
  • Proglucagon
  • Gastrin-Releasing Peptide
  • Glucagon-Like Peptide 1
  • Glucagon
  • Arginine
  • Glucose