Identification and Characterization of the Protein Encoded by the Human C-Myb Proto-Oncogene

EMBO J. 1986 Aug;5(8):1903-11.

Abstract

We have identified the product of the human c-myb proto-oncogene as a 80,000-Mr protein, p80c-myb, by using polyclonal and monoclonal antibodies raised against a bacterially synthesized polypeptide from the amino terminus of the viral myb protein. p80c-myb shares at least two distinct antigenic sites with the amino terminal region of the v-myb protein. p80c-myb is found only in hematopoietic cells or in cells that contain amplified c-myb genes. Like the chicken myb proteins, p80c-myb is a nuclear DNA-binding protein that is predominantly associated with chromatin and exhibits a short half-life of approximately 1 hour.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Avian Myeloblastosis Virus / genetics
  • Cell Line
  • Cell Transformation, Neoplastic
  • Chickens
  • Colonic Neoplasms
  • DNA-Binding Proteins / genetics
  • Escherichia coli / genetics
  • Genes
  • Genetic Vectors
  • Half-Life
  • Humans
  • Hybridomas / immunology
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins / isolation & purification
  • Proto-Oncogene Proteins c-myb
  • Proto-Oncogenes*
  • Rabbits

Substances

  • Antibodies, Monoclonal
  • DNA-Binding Proteins
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-myb