Immobilization of Ochrobactrum sp. on Biochar/Clay Composite Particle: Optimization of Preparation and Performance for Nitrogen Removal

Pengfei Sun; Xiao Huang; Yixiao Xing; Wenlong Dong; Jianghua Yu; Jie Bai; Weiyan Duan

doi:10.3389/fmicb.2022.838836

Immobilization of Ochrobactrum sp. on Biochar/Clay Composite Particle: Optimization of Preparation and Performance for Nitrogen Removal

Front Microbiol. 2022 Mar 2:13:838836. doi: 10.3389/fmicb.2022.838836. eCollection 2022.

Authors

Pengfei Sun^{1

2

3}, Xiao Huang⁴, Yixiao Xing⁴, Wenlong Dong⁵, Jianghua Yu⁴, Jie Bai⁶, Weiyan Duan⁷

Affiliations

¹ Ministry of Natural Resources, Fourth Institute of Oceanography, Beihai, China.
² Key Laboratory of Tropical Marine Ecosystem and Bioresource, Ministry of Natural Resources, Beihai, China.
³ Guangxi Beibu Gulf Key Laboratory of Marine Resources, Environment and Sustainable Development, Beihai, China.
⁴ Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, Nanjing, China.
⁵ Shandong Marine Forecast and Hazard Mitigation Service, Qingdao, China.
⁶ College of Environmental Science and Engineering, Ocean University of China, Qingdao, China.
⁷ Ocean College of Hebei Agricultural University, Qinhuangdao, China.

Abstract

The objective of this study was to prepare biochar/clay composite particle (BCCP) as carrier to immobilize Ochrobactrum sp. to degrade ammonia nitrogen (NH₄ ⁺-N), and the effects of calcined program and immobilizing material were investigated. Results reflected that the parameters were as follows: calcined temperature 400°C, heating rate 20°C min^-1, and holding time 2 h, and the adsorption capacity could reach 0.492 mg g^-1. Sodium alginate/polyvinyl alcohol, as embedding material, jointed with NH₄ ⁺-N adsorption process and then degraded by Ochrobactrum sp. with 79.39% degradation efficiency at 168 h. Immobilizing Ochrobactrum sp. could protect strain from high salt concentration to achieve the exceeding degradation efficiency than free bacteria, but could not block the impact of low temperature.

Keywords: Ochrobactrum sp.; ammonia-oxidizing bacteria; biochar/clay composite particle; calcined temperature; immobilization.