Immunopotentiative effect of polysaccharide from kefir grain, KGF-C, administered orally in mice

Immunopharmacology. 1986 Aug;12(1):29-35. doi: 10.1016/0162-3109(86)90049-4.


Since a water-soluble polysaccharide (KGF-C) from the kefir grains was shown to have the property of retarding tumor growth in vivo when administered orally, the effect of KGF-C was examined on antibody responses to thymus-dependent antigen, sheep red blood cells (SRBC), and thymus-independent antigen, dinitrophenyl-Ficoll and trinitrophenyl-lipopolysaccharide. Antibody response in mice intubated with KGF-C was enhanced to low doses of SRBC, but not to optimal or high doses. The optimal dose of KGF-C required for the enhancement was 100 mg/kg body weight. The time-course studies on KGF-C administration implied that KGF-C exerted its effect on the early events of anti-SRBC response. The enhancement was not due to the alteration of kinetics of anti-SRBC responses. Furthermore, the enhancing effect on antibody responses to thymus-independent antigens, such as dinitrophenyl-Ficoll and trinitrophenyl-lipopolysaccharide, was observed neither in nu/nu nor in nu/+ mice, and the effect on delayed-type hypersensitivity response to a low dose of SRBC in normal mice was also found. These findings suggest that the oral immune enhancement by KGF-C is elucidated probably through T-cell but not through B-cell participation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic*
  • Administration, Oral
  • Animals
  • Dose-Response Relationship, Drug
  • Hemolytic Plaque Technique
  • Hypersensitivity, Delayed*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred Strains
  • Mice, Nude
  • Polysaccharides / administration & dosage
  • Polysaccharides / metabolism
  • Polysaccharides / pharmacology*


  • Adjuvants, Immunologic
  • Polysaccharides
  • kefir grain polysaccharide