Regional differences in 5-hydroxytryptamine and catecholamine uptake in primary astrocyte cultures

J Neurochem. 1986 Nov;47(5):1647-52. doi: 10.1111/j.1471-4159.1986.tb00808.x.


The uptake of 3H-labelled 5-hydroxytryptamine (5-HT, serotonin) norepinephrine ([3H]NE), and 3,4-dihydroxyphenylethylamine ([ 3H]dopamine, [3H]DA) was studied in primary astrocyte cultures prepared from the cerebral cortex, corpus striatum, and hippocampal regions of neonatal rat brain. Na+-dependent uptake showed marked regional differences. For [3H]5-HT the magnitude of uptake was corpus striatum greater than or equal to cerebral cortex greater than hippocampus, whereas for [3H]NE the order was hippocampus greater than corpus striatum greater than cerebral cortex. For [3H]DA, only the hippocampal cultures showed significant Na+-dependent uptake. [3H]5-HT uptake was specifically inhibited by 10(-7) M fluoxetine whereas [3H]NE uptake was preferentially inhibited by 10(-7) M desipramine. These results may reflect regional brain specialization and/or different developmental patterns of high affinity uptake of serotonin and catecholamines by astrocytes in situ.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Newborn / metabolism
  • Astrocytes / metabolism*
  • Catecholamines / metabolism*
  • Cells, Cultured
  • Dopamine / metabolism
  • Fluoxetine / pharmacology
  • Glial Fibrillary Acidic Protein / analysis
  • Histocytochemistry
  • Microscopy, Phase-Contrast
  • Norepinephrine / metabolism
  • Rats
  • Serotonin / metabolism*
  • Tissue Distribution


  • Catecholamines
  • Glial Fibrillary Acidic Protein
  • Fluoxetine
  • Serotonin
  • Dopamine
  • Norepinephrine