Development of a device for cultivation and isolation of microbes using a specialized cellulose film

Hideki Aoyagi; Ryo Saitoh; Koichi Murayama

doi:10.1016/j.mimet.2022.106450

Development of a device for cultivation and isolation of microbes using a specialized cellulose film

J Microbiol Methods. 2022 Apr:195:106450. doi: 10.1016/j.mimet.2022.106450. Epub 2022 Mar 19.

Authors

Hideki Aoyagi¹, Ryo Saitoh², Koichi Murayama³

Affiliations

¹ Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan; Agro-Biological Resource Sciences, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan. Electronic address: aoyagi.hideki.ge@u.tsukuba.ac.jp.
² Agro-Biological Resource Sciences, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan.
³ Futamura Chemical Co., Ltd., 2-29-16, Meieki, Nakamura-ku, Nagoya-shi, Aichi Prefecture 450-0002, Japan.

PMID: 35318086
DOI: 10.1016/j.mimet.2022.106450

Abstract

A device was developed for the cultivation and isolation of microbes using a specialized cellulose film (SCF). This method can solve problems associated with the agar plate method. It can also be used for other culture-related operations, which is not possible with the traditional agar plate culture method.

Keywords: Cellulose film; Culture device; Isolation; Liquid medium; Microbe.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agar
Cellulose*
Culture Media

Substances

Culture Media
Agar
Cellulose