In this communication, we report our findings on the stability of unsubstituted BOPHY in different media. Our studies showed that unsubstituted BOPHY is highly unstable in water, methanol, and DMSO. The dye was observed to be stable in aprotic and/or relatively apolar solvents (ACN, acetone, DCM, and toluene) for a routine application. The pH of the medium was shown to affect the stability of the dye dramatically. Herein, we also presented a one-pot procedure for the synthesis of the dye.
Keywords: Aqueous solution; BOPHY; Chemical stability; Fluorophore stability; One-pot synthesis.
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