We report an outbreak of severe acute respiratory syndrome coronavirus 2 involving 3 Malayan tigers (Panthera tigris jacksoni) at a zoo in Tennessee, USA. Investigation identified naturally occurring tiger-to-tiger transmission; genetic sequence change occurred with viral passage. We provide epidemiologic, environmental, and genomic sequencing data for animal and human infections.
Keywords: COVID-19; Malayan tigers; SARS; SARS-CoV-2; Tennessee; United States; coronavirus; coronavirus disease; outbreak investigation; respiratory infections; severe acute respiratory syndrome coronavirus 2; viruses; zoonoses.