Neuronal Microsurgery with an Yb-Doped Fiber Femtosecond Laser

doi:10.1007/978-1-0716-2181-3_17

. 2022:2468:319-328.

doi: 10.1007/978-1-0716-2181-3_17.

Neuronal Microsurgery with an Yb-Doped Fiber Femtosecond Laser

Maria B Harreguy¹, Tracy S Tran¹, Gal Haspel²

Affiliations

¹ Federated Department of Biological Sciences, New Jersey Institute of Technology and Rutgers University, Newark, NJ, USA.
² Federated Department of Biological Sciences, New Jersey Institute of Technology and Rutgers University, Newark, NJ, USA. haspel@njit.edu.

PMID: 35320573
DOI: 10.1007/978-1-0716-2181-3_17

Neuronal Microsurgery with an Yb-Doped Fiber Femtosecond Laser

Maria B Harreguy et al. Methods Mol Biol. 2022.

. 2022:2468:319-328.

doi: 10.1007/978-1-0716-2181-3_17.

Authors

Maria B Harreguy¹, Tracy S Tran¹, Gal Haspel²

Affiliations

¹ Federated Department of Biological Sciences, New Jersey Institute of Technology and Rutgers University, Newark, NJ, USA.
² Federated Department of Biological Sciences, New Jersey Institute of Technology and Rutgers University, Newark, NJ, USA. haspel@njit.edu.

PMID: 35320573
DOI: 10.1007/978-1-0716-2181-3_17

Abstract

Laser microsurgery allows the user to ablate cell bodies or disconnect nerve fibers by using a laser microbeam focused through a microscope. This technique was pioneered in C. elegans where it led to exciting discoveries in the fields of development and neurobiology. All neurons studied so far in C. elegans can regenerate and regrow axons and dendrites after injury, allowing studies of the molecular and cellular basis of neuroregeneration. In this chapter, we describe how to assemble and operate a platform for Yb-doped fiber laser microsurgery. The novel laser setup described here is a more robust, lower cost, and user-friendly alternative to other femtosecond-pulsed laser systems.

Keywords: Axotomy; Dendrotomy; Injury; Laser; Microscopy; Microsurgery; Regeneration.

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Cited by

Semaphorin signaling restricts neuronal regeneration in C. elegans.
Harreguy MB, Tanvir Z, Shah E, Simprevil B, Tran TS, Haspel G. Harreguy MB, et al. Front Cell Dev Biol. 2022 Oct 17;10:814160. doi: 10.3389/fcell.2022.814160. eCollection 2022. Front Cell Dev Biol. 2022. PMID: 36325362 Free PMC article.

References

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1. Yanik MF, Cinar H, Cinar HN, Chisholm AD, Jin Y, Ben-Yakar A (2004) Neurosurgery: functional regeneration after laser axotomy. Nature 432:822. https://doi.org/10.1038/432822a - DOI - PubMed
1. DiLoreto EM, Chute CD, Bryce S, Srinivasan J (2019) Novel technological advances in functional connectomics in C. elegans. J Dev Biol 7:doi:10.3390/jdb7020008 - DOI

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[1] Sulston JE, White JG (1980) Regulation and cell autonomy during postembryonic development of Caenorhabditis elegans. Dev Biol 78:577–597. https://doi.org/10.1016/0012-1606(80)90353-X - DOI - PubMed

[2] Sulston JE, White JG (1980) Regulation and cell autonomy during postembryonic development of Caenorhabditis elegans. Dev Biol 78:577–597. https://doi.org/10.1016/0012-1606(80)90353-X - DOI - PubMed

[3] Chalfie M, Sulston JE, White JG, Southgate E, Thomson JN, Brenner S (1985) The neural circuit for touch sensitivity in Caenorhabditis elegans. J Neurosci 5:956–964. https://doi.org/10.1523/JNEUROSCI.05-04-00956.1985 - DOI - PubMed - PMC

[4] Chalfie M, Sulston JE, White JG, Southgate E, Thomson JN, Brenner S (1985) The neural circuit for touch sensitivity in Caenorhabditis elegans. J Neurosci 5:956–964. https://doi.org/10.1523/JNEUROSCI.05-04-00956.1985 - DOI - PubMed - PMC

[5] McIntire SL, Jorgensen E, Kaplan J, Horvitz HR (1993) The GABAergic nervous system of Caenorhabditis elegans. Nature 364:337–341. https://doi.org/10.1038/364337a0 - DOI - PubMed

[6] McIntire SL, Jorgensen E, Kaplan J, Horvitz HR (1993) The GABAergic nervous system of Caenorhabditis elegans. Nature 364:337–341. https://doi.org/10.1038/364337a0 - DOI - PubMed

[7] Yanik MF, Cinar H, Cinar HN, Chisholm AD, Jin Y, Ben-Yakar A (2004) Neurosurgery: functional regeneration after laser axotomy. Nature 432:822. https://doi.org/10.1038/432822a - DOI - PubMed

[8] Yanik MF, Cinar H, Cinar HN, Chisholm AD, Jin Y, Ben-Yakar A (2004) Neurosurgery: functional regeneration after laser axotomy. Nature 432:822. https://doi.org/10.1038/432822a - DOI - PubMed

[9] DiLoreto EM, Chute CD, Bryce S, Srinivasan J (2019) Novel technological advances in functional connectomics in C. elegans. J Dev Biol 7:doi:10.3390/jdb7020008 - DOI

[10] DiLoreto EM, Chute CD, Bryce S, Srinivasan J (2019) Novel technological advances in functional connectomics in C. elegans. J Dev Biol 7:doi:10.3390/jdb7020008 - DOI

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Neuronal Microsurgery with an Yb-Doped Fiber Femtosecond Laser

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Neuronal Microsurgery with an Yb-Doped Fiber Femtosecond Laser

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