RTBV-Based VIGS Vector for Functional Genomics in Rice: Methodology, Advances, Challenges, and Future Implications

Gaurav Kumar; Kamlesh Kumari; Indranil Dasgupta

doi:10.1007/978-1-0716-1875-2_8

RTBV-Based VIGS Vector for Functional Genomics in Rice: Methodology, Advances, Challenges, and Future Implications

Methods Mol Biol. 2022:2408:117-131. doi: 10.1007/978-1-0716-1875-2_8.

Authors

Gaurav Kumar¹, Kamlesh Kumari¹, Indranil Dasgupta²

Affiliations

¹ Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, India.
² Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, India. indasgup@south.du.ac.in.

PMID: 35325420
DOI: 10.1007/978-1-0716-1875-2_8

Abstract

The availability of protocols for virus-induced gene silencing (VIGS) in rice has opened up an important channel for the elucidation of gene functions in this important crop plant. Here, we present an updated protocol of a VIGS system based on Rice tungro bacilliform virus (RTBV) for gene silencing in rice. We present complete updated protocols for VIGS in rice, compare the system with other existing ones for monocots, identify some of the challenges faced by this system and discuss ways in which the vector could be improved for better silencing efficiency.

Keywords: Agrobacterium tumefaciens; Agroinoculation; Monocot VIGS; Rice tungro bacilliform virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Silencing
Genomics
Oryza* / genetics
Tungrovirus* / genetics