5,6-Epoxyeicosatrienoic acid mobilizes Ca2+ in anterior pituitary cells

Biochem Biophys Res Commun. 1986 Sep 30;139(3):1188-94. doi: 10.1016/s0006-291x(86)80303-5.


Luteinizing hormone releasing hormone stimulates the concomitant release of luteinizing hormone and 45Ca2+ from prelabeled anterior pituitary cells. Indomethacin (10 microM) and nordihydroguaiaretic acid (10 microM) had no effect on the luteinizing hormone releasing hormone-stimulated release of either luteinizing hormone or 45Ca2+. Eicosatetraynoic acid (10 microM) blocked both luteinizing hormone releasing hormone-stimulated luteinizing hormone secretion and luteinizing hormone releasing hormone-stimulated 45Ca2+ efflux. 5,6-Epoxyeicosatrienoic acid stimulated both luteinizing hormone secretion and 45Ca2+ efflux from anterior pituitary cells. Additionally, 5,6-epoxyeicosatrienoic acid closely mimics the ability of luteinizing hormone releasing hormone to increase intracellular free calcium. These results are consistent with the hypothesis that 5,6-EET alters calcium homeostasis in a manner similar to that observed during luteinizing hormone releasing hormone stimulation of luteinizing hormone release.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 8,11,14-Eicosatrienoic Acid / analogs & derivatives
  • 8,11,14-Eicosatrienoic Acid / metabolism*
  • Animals
  • Calcium / metabolism*
  • Cytosol / metabolism
  • Ethers / pharmacology
  • Fatty Acids, Unsaturated / metabolism*
  • Gonadotropin-Releasing Hormone / pharmacology
  • Indomethacin / pharmacology
  • Ionomycin
  • Luteinizing Hormone / metabolism
  • Male
  • Masoprocol / pharmacology
  • Pituitary Gland, Anterior / drug effects*
  • Pituitary Gland, Anterior / metabolism
  • Rats
  • Rats, Inbred Strains


  • Ethers
  • Fatty Acids, Unsaturated
  • Gonadotropin-Releasing Hormone
  • Ionomycin
  • Masoprocol
  • 5,6-epoxy-8,11,14-eicosatrienoic acid
  • Luteinizing Hormone
  • 8,11,14-Eicosatrienoic Acid
  • Calcium
  • Indomethacin