Development of functional antibodies against influenza B virus by activation-induced cytidine deaminase in hybridoma cells

Virol Sin. 2022 Aug;37(4):619-622. doi: 10.1016/j.virs.2022.03.009. Epub 2022 Mar 22.

¹ National Institute of Diagnostics and Vaccine Development in Infectious Diseases, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health & School of Life Science, Xiamen University, Xiamen 361102, China.
² National Institute of Diagnostics and Vaccine Development in Infectious Diseases, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health & School of Life Science, Xiamen University, Xiamen 361102, China; Biosafety Level-3 Laboratory, School of Public Health, Southern Medical University, Guangzhou 510515, China. Electronic address: a124965468@smu.edu.cn.
³ National Institute of Diagnostics and Vaccine Development in Infectious Diseases, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health & School of Life Science, Xiamen University, Xiamen 361102, China. Electronic address: yxchen2008@xmu.edu.cn.
⁴ National Institute of Diagnostics and Vaccine Development in Infectious Diseases, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health & School of Life Science, Xiamen University, Xiamen 361102, China. Electronic address: wxluo@xmu.edu.cn.

Abstract

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Class-switch recombination was mimicked in hybridomas by controllable expression of activation-induced cytidine deaminase.
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IgG antibodies were generated through this system in an anti-Flu B IgM hybridoma 7G1.
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IgG1 and IgG2a subtypes of 7G1 present improved antiviral activity in vitro and in vivo.