Isolation of the yeast calmodulin gene: calmodulin is an essential protein

Cell. 1986 Nov 7;47(3):423-31. doi: 10.1016/0092-8674(86)90599-4.

Abstract

Calmodulin was purified from Saccharomyces cerevisiae based on its characteristic properties. Like other calmodulins, the yeast protein is small, heat-stable, acidic, retained by hydrophobic matrices in a Ca2+-dependent manner, exhibits a pronounced Ca2+-induced shift in electrophoretic mobility, and binds 45Ca2+. Using synthetic oligonucleotide probes designed from the sequences of two tryptic peptides derived from the purified protein, the gene encoding yeast calmodulin was isolated. The gene (designated CMD1) is a unique, single-copy locus, contains no introns, and resides on chromosome II. The amino acid sequence of yeast calmodulin shares 60% identity with other calmodulins. Disruption or deletion of the yeast calmodulin gene results in a recessive-lethal mutation; thus, calmodulin is essential for the growth of yeast cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Calcium / metabolism
  • Calmodulin / genetics*
  • Calmodulin / isolation & purification
  • DNA, Fungal / isolation & purification*
  • Genes, Lethal
  • Saccharomyces cerevisiae / genetics*

Substances

  • Calmodulin
  • DNA, Fungal
  • Calcium

Associated data

  • GENBANK/M14760