Background: Vascular cell adhesion molecule (VCAM-1) mediates pulpitis via regulating interleukin (IL)-1β. microRNA (miR)-126 was reported to regulate the VCAM-1 under many different pathophysiological circumstances. We investigated variations of miR-126 and VCAM-1 in inflamed patient pulp tissues and determined potential roles of miR-126 in pulpitis using human dental pulp cells (hDPCs) in vitro.
Methods: We quantitatively measured the transcripts of miR-126 and VCAM-1 in inflamed human pulp tissues using qRT-PCR and compared with those from healthy human pulp tissues. In addition, we transfected miR-126 in hDPCs using plasmid DNA (pDNA)-encoding miR-126 delivered by polyethylenimine (PEI) nanoparticles.
Results: The irreversible pulpitis significantly reduced miR-126 and increased the transcript of VCAM-1 in pulp tissues (p < 0.05). pDNA-encoding miR-126 delivered PEI nanoparticles and effectively upregulated the expression of miR-126 in hDPCs (p < 0.05). The overexpression of miR-126 could effectively suppress the transcripts and protein levels of VCAM-1 and IL-1β induced by Pg-LPS at 100ng/mL in DPCs (p < 0.05).
Conclusions: miR-126 is involved in pulpitis and downregulated the VCAM-1 and IL-1β in DPCs. miR-126 may be a potential target to attenuate the inflammation of pulpitis.
Keywords: microRNA-126; human dental pulp cells; inflammation; interleukin-1β; vascular cell adhesion molecule-1.
© 2022 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.