Meta-Analysis of Randomized Clinical Trials Evaluating Effectiveness of a Multivitamin Supplementation against Oxidative Stress in Healthy Subjects

Nutrients. 2022 Mar 10;14(6):1170. doi: 10.3390/nu14061170.


A meta-analysis has been widely applied to draw general conclusions using a set of studies with similar purposes and designs. This study aimed to perform a meta-analysis of six randomized placebo-controlled trials, independently conducted for the relationship between a plant-based multivitamin/mineral supplementation (PMS) and oxidative stress for 6 to 8 weeks, to provide overall estimates of those effects. In detail, linear mixed model analysis was first conducted on each study to obtain individual estimates; then, two types of meta-analysis were applied to combine the individual estimates from all available studies (overall meta-analysis) and region-specific studies (subgroup meta-analysis). In the meta-analysis, we selected 19 biomarker variables that overlapped in at least two studies and found 6 variables significant in at least one meta-analysis. The overall estimates of beta coefficients were 0.17 for vitamin C, 0.80 for vitamin B6, 0.46 for vitamin B12, 0.81 for folate, 0.36 for β-carotene, and -0.17 for oxidized LDL (ox-LDL). Subsequent association analysis revealed significant negative correlations between plasma free radical scavenging nutrients and plasma ox-LDL levels, indicating a general benefit of PMS in alleviating oxidative stress by providing exogenous oxidant scavengers.

Keywords: exogenous oxidant scavenger; meta-analysis; multivitamin supplementation; oxidative damage.

Publication types

  • Meta-Analysis

MeSH terms

  • Dietary Supplements*
  • Healthy Volunteers
  • Humans
  • Oxidative Stress
  • Randomized Controlled Trials as Topic
  • Vitamins* / pharmacology
  • Vitamins* / therapeutic use


  • Vitamins