Study on synergistic effects of carboxymethyl cellulose and LIPUS for bone tissue engineering

Liang Tang; Tianpei Wu; Yaling Zhou; Yi Zhong; Lijun Sun; Jianzhong Guo; Xiushan Fan; Dean Ta

doi:10.1016/j.carbpol.2022.119278

Study on synergistic effects of carboxymethyl cellulose and LIPUS for bone tissue engineering

Carbohydr Polym. 2022 Jun 15:286:119278. doi: 10.1016/j.carbpol.2022.119278. Epub 2022 Feb 22.

Authors

Liang Tang¹, Tianpei Wu¹, Yaling Zhou¹, Yi Zhong¹, Lijun Sun¹, Jianzhong Guo², Xiushan Fan³, Dean Ta⁴

Affiliations

¹ Institute of Sports Biology, Shaanxi Normal University, Xi'an, China.
² Shaanxi Key Laboratory of Ultrasonics, Shaanxi Normal University, Xi'an, China.
³ Institute of Sports Biology, Shaanxi Normal University, Xi'an, China. Electronic address: xshfan@snnu.edu.cn.
⁴ Department of Electronic Engineering, Fudan University, Shanghai, China; Key Laboratory of Medical Imaging Computing and Computer Assisted Intervention (MICCAI) of Shanghai, Shanghai, China. Electronic address: tda@fudan.edu.cn.

PMID: 35337527
DOI: 10.1016/j.carbpol.2022.119278

Abstract

In this research, the pharmaceutical used carboxymethyl cellulose (CMC) was successfully synthesized by ramie fiber and sodium monochloroacetate. Meanwhile, this study focused on the proliferation and differentiation of osteoblast which was stimulated by ramie based CMC. Additionally, the synergistic effects between CMC and Low-intensity pulsed ultrasound (LIPUS) for the proliferation and differentiation of osteoblast was also evaluated in this study. The experimental results demonstrated that ramie based CMC were nontoxic and cytocompatible in MC3T3-E1 cells culture; the dose of 300 μg L^-1 CMC applied in this work displayed better efficiency of promoting the osteogenic proliferation and differentiation. Meanwhile, the results were also exhibited that the combination of CMC and LIPUS (1.5 MHz, 80 mW cm^-2) has a significant proliferation and differentiation promoting effects compared with the single factor intervention. This study provides a new pathway for bio-polymeric materials in the repair and regeneration of the bone tissues.

Keywords: Carboxymethyl cellulose; Low-intensity pulsed ultrasound; Osteoblast; Proliferation and differentiation; Ramie fiber.

MeSH terms

Bone and Bones
Carboxymethylcellulose Sodium* / pharmacology
Cell Differentiation
Osteogenesis
Tissue Engineering*
Ultrasonic Waves

Substances

Carboxymethylcellulose Sodium