Efficient extraction of chitin from crustacean waste via a novel ternary natural deep eutectic solvents

Yuehai Wang; Yongqing Yang; Rui Wang; Yanlin Zhu; Pengbo Yang; Zhangnan Lin; Zhihui Wang; Wei Cong

doi:10.1016/j.carbpol.2022.119281

Efficient extraction of chitin from crustacean waste via a novel ternary natural deep eutectic solvents

Carbohydr Polym. 2022 Jun 15:286:119281. doi: 10.1016/j.carbpol.2022.119281. Epub 2022 Feb 25.

Authors

Yuehai Wang¹, Yongqing Yang¹, Rui Wang¹, Yanlin Zhu¹, Pengbo Yang², Zhangnan Lin², Zhihui Wang³, Wei Cong⁴

Affiliations

¹ State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; School of Chemical and Engineering, University of Chinese Academy of Sciences, Beijing 100049, China.
² State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; Nanjing IPE Institute of Green Manufacturing Industry, Nanjing 211100, China.
³ State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
⁴ State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: weicong@ipe.ac.cn.

PMID: 35337528
DOI: 10.1016/j.carbpol.2022.119281

Abstract

Extraction of chitin from crustacean waste with acidic natural deep eutectic solvents (NADESs) is usually accompanied by degradation of chitin, which lowers the yield and molecular weight of product. Herein, this study proposed a eco-friendly and feasible route for effectively improving the yield and molecular weight of chitin by introducing N-acetyl-D-glucosamine into ternary NADESs. A high molecular weight chitin with molecular weight of 3.92 × 10⁵ Da, purity of 90.2% and yield of 85.6% was obtained from crab shell. Compared with conventional acid/alkali and binary NADESs method, the maximum yield of chitin extracted by ChCl-G-FA2 was increased by 1.57 times and 1.39 times respectively. Molecular weight of chitin was 3.16 times that of acid/alkali method. Recycling performance evaluation revealed that the purity of chitin could still reach 80.4% after five cycles of NADESs. This study provided a eco-friendly utilization strategy for crustacean waste based on multifunctional NADESs.

Keywords: Biopolymer chitin; Crustacean waste; Eco-friendly extraction; High molecular weight; High yield; Natural deep eutectic solvents.

MeSH terms

Animals
Chitin*
Crustacea
Deep Eutectic Solvents*
Solvents

Substances

Deep Eutectic Solvents
Solvents
Chitin