ELISA using whole Legionella pneumophila cell as antigen. Comparison between monovalent and polyvalent antigens for the serodiagnosis of human legionellosis

J Immunol Methods. 1986 Oct 23;93(1):77-81. doi: 10.1016/0022-1759(86)90435-7.

Abstract

An indirect enzyme-linked immunosorbent assay (ELISA) using the six serogroups of whole L. pneumophila bound to microtitre plate wells is described for the serodiagnosis of legionellosis. Comparative studies using monovalent antigen indicated a high correlation between ELISA and indirect immunofluorescence antibody (IFA) tests (r = 0.90, P less than 0.001). Testing 196 human sera by ELISA using both monovalent and polyvalent antigens have established the efficiency of the polyvalent antigen for screening purposes. The ELISA test system exhibited rapidity, sensitivity and reproducibility and should be considered as an alternative to the IFA test for routine serodiagnosis of legionellosis.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Antibodies, Bacterial / analysis*
  • Antigens, Bacterial / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Fluorescent Antibody Technique
  • Follow-Up Studies
  • Humans
  • Legionella / immunology
  • Legionnaires' Disease / diagnosis*
  • Male
  • Reference Values
  • Serologic Tests

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial