Leclercia pneumoniae sp. nov., a bacterium isolated from clinical specimen in Leipzig, Germany

Int J Syst Evol Microbiol. 2022 Mar;72(3). doi: 10.1099/ijsem.0.005293.


Strain 49125T was isolated from an infant with pneumonia and septicaemia at the Leipzig University Hospital. Phenotypic and genomic traits were investigated. The strain's biochemical profile and its MALDI-TOF spectrogram did not differ from comparative samples of Leclercia adecarboxylata, thus far the sole member of the Leclercia species. A circular genome with a size of 4.4 Mbp and a G+C content of 55.0 mol% was reconstructed using hybrid Illumina and Nanopore sequencing. Phylogenetic analysis was based on 172 marker genes and validated using a k-mer-based search against a large genome collection including subsequent in silico DNA-DNA hybridization. Whole genome average nucleotide identity to any described species was below 95%, suggesting that strain 49125T represents a new species, for which we propose the name Leclercia pneumoniae sp. nov. with the type strain 49125T (=LMG 32245T=DSM 112336T).

Keywords: Enterobacterales; Leclercia; next-generation sequencing.

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Enterobacteriaceae / genetics
  • Fatty Acids* / chemistry
  • Humans
  • Phylogeny
  • Pneumonia*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA


  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S